nk 92 mi cell line  (ATCC)

Journal: bioRxiv

Article Title: CellTrap: A Microfluidic Platform Enabling Cell-Cell Interactions at Variable Effector to Target Ratios

doi: 10.64898/2025.12.25.696500

Figure Lengend Snippet: (A) The experimental loading frequencies ( f ) of individual particles, i.e., green and red, are compared to the theoretical Poisson distributions ( P ) for λ 1 = 0.51 (green) and λ 2 = 0.54 (red). (B) A combinatorial loading distribution of the two particle types was analyzed using a double Poisson distribution, which is compared with the experimental dual loading frequency. (C) Representative bright-field, fluorescence, and overlay images of red and green particles trapped in a channel at different k 1 : k 2 ratios. (D) The experimental loading frequencies ( f ) and the theoretical Poisson distributions ( P ) are plotted for the U87 GFP and NK92 IL2 cells seeded in the CellTrap device with λ 1 = 0.83 (U87 GFP ) and λ 2 = 0.58 (NK92 IL2 ). (E) The experimental dual loading frequency and theoretical double Poisson distributions result in varying E:T or k 1 : k 2 ratios. (F) Representative bright-field, fluorescence, and overlay images of cancer (purple) and immune (white arrows) cells trapped inside a channel at different E:T ratios. (scale bars: 30 μm)

Article Snippet: The human cell lines NK92 IL2 , U87, K562, and LS174T were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence

Journal: bioRxiv

Article Title: CellTrap: A Microfluidic Platform Enabling Cell-Cell Interactions at Variable Effector to Target Ratios

doi: 10.64898/2025.12.25.696500

Figure Lengend Snippet: (A) Fluorescence intensity of U87 GFP cells decreases significantly after 4 h of co-incubation with PBMCs at E:T = ≥1:≥1. This data is curated from two independent CellTrap devices (N = 2), where, in total, 97 traps were analyzed (n = 97). (B) Inside one of the CellTrap devices used in (A), control traps with only one U87 GFP cell per trap, i.e., E:T = 0:1, were analyzed, maintaining fluorescence signal over 14 h (N = 1, n = 18). (C) Representative images of U87 GFP cells interacting with PBMCs at different E:T ratios, along with the control group containing only U87 GFP cells. (D) Fluorescence intensity of U87 GFP cells decreases significantly after 4 h of co-incubation with NK92 IL2 at E:T = 1:1. This data is curated from four independent CellTrap devices (N = 4), where, in total, 213 traps with E:T = 1:1 were analyzed (n = 213). (E) Inside one of the CellTrap devices used in (D), control traps with only one U87 GFP cell per trap, i.e., E:T = 0:1, were analyzed, maintaining a fluorescence signal over 14 h (N = 1, n = 50). (F) Representative images of U87 GFP cells interacting with NK92 IL2 at different E:T ratios, along with the control group containing only U87 GFP cells. (G) Fluorescence intensity of U87 GFP at 0 h and 14 h of co-incubation with NK92 IL2 at E:T = 1:1, 1:2, 2:1 and 2:2. The intensity drop is significant in all E:T ratios except 1:2. This data is curated from the same CellTrap devices used in (D).

Article Snippet: The human cell lines NK92 IL2 , U87, K562, and LS174T were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Incubation, Control

Journal: bioRxiv

Article Title: CellTrap: A Microfluidic Platform Enabling Cell-Cell Interactions at Variable Effector to Target Ratios

doi: 10.64898/2025.12.25.696500

Figure Lengend Snippet: (A) Calcium flux (normalized intensity) in NK92 IL2 immune cells varies over time in the presence of various cancer cell lines (U87, LS174T, K562). NK92 IL2 cells alone show a flat response (control). Each grey line represents a single immune cell tracked. Representative images show NK92 IL2 cells (Green) and cancer cells (U87, LS174T, K562) co-incubated in the CellTrap chip at an E:T ratio of 1:1. n = number of traps analyzed. (B) The killing response of NK92 IL2 cells at different E:T ratios (1:1, 1:2, 2:1) against cancer cells (U87, LS174T, K562) is quantified and compared with control groups with E:T ratios of ≥1:0 and 0:≥1. N = number of CellTrap chips analyzed. n = number of traps analyzed. Representative images show NK92 IL2 cells interacting with cancer cells (U87, LS174T, K562 in blue) with varying E:T ratios at 0 and 14 hours. Red color indicates cell death at 14 hours. Scale bars: 25µm.

Article Snippet: The human cell lines NK92 IL2 , U87, K562, and LS174T were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control, Incubation